[2012.3.15] Our first paper with the title "Targeted gene engineering inClostridium cellulolyticumH10 without methylation" is published onJ. Microbiol. Methodsnow. The paper describes our progresses in the genetic manipulation ofClostridium cellulolyticumH10. A methylation-free genetic platform was developed with  high transformation efficiency, screening rate and stability.The mutant H10DmspIlostMspI activity and accepted unmethylated DNA efficiently, which is capable of heterologous gene expression and serial gene inactivation. Further, an anaerobic green fluorescent reporter system was constructed in H10DmspI. This genetic platform ofC. cellulolyticumH10 will facilitate the metabolic engineering ofC. cellulolyticumaiming at faster cellulose degradation and higher biofuel production at the molecular level.

The first author Gu-zhen Cui is a Ph.D. candidate student. The Metabolomics Group leader Prof. Dr. Qiu Cui and the Assistant Professor Dr. Ya-Jun Liu in the group are corresponding authors. This work was supported by One-hundred-Talented-People program (KSCX2-YW-G-066) from Chinese Academy of Sciences and National Basic Research Program of China (973-project, Grant No. 2011CB707404).

NCBI PubMed

Gu-zhen Cui, Wei Hong, Jie Zhang, Wen-li Li, Yingang Feng, Ya-jun Liu*, and Qiu Cui* (2012) Targeted gene engineering inClostridium cellulolyticumH10 without methylation.J. Microbiol. Methods89, 201-208.Full Text Link (Publisher website)