[2015.2.15] We publish two papers about protein NMR assignments onBiomol. NMR Assign.The first paper is the NMR assigments of translationally-controlled tumor protein (TCTP) from photosynthetic microalgaNannochloropsis oceanicapublished online on Feb. 14. TCTP is a eukaryote-conserved growth-related protein whose functions in plant were rarely reported.N. oceanicais a photosynthetic microalga with high yield of lipid and high-value polyunsaturated fatty acid, which is promising for biodiesel production. Study of growth-related proteins may provide new clue for improving the yield of lipid. TCTP from N. oceanica shares low sequence identity with structure-known TCTPs. The paper reported the NMR resonance assignments of TCTP from N. oceanica for further structural and functional studies. The second paper online on Feb. 15 is about the NMR assigments of the periplasmic domain of a cellulose-sensing trans-membrane anti-sigma factor fromClostridium thermocellum. The anti-sigma factor and corresponding sigma factor are regulators for cellulosome expression. The anti-sigma factor is a membrane protein containing an N-terminal intracellular domain, a trans-membrane helix, a periplasmic domain, a proline-rich region which is probably required for crossing the cell wall, and a C-terminal carbohydrate-binding domain or glycoside hydrolase domain. The periplasmic domain may play a key role in signal transduction; however, its three-dimensional structure is still unknown. The paper reported the NMR resonance assignments of the periplasmic domain of anti-sigma factor RsgI2 from C. thermocellum as a basis for further structural determination and functional studies.

NCBI PubMed 

1. Xiaoke Ding, Chao Chen, Qiu Cui, Wenli Li* and Yingang Feng* (2015) Resonance assignments of the periplasmic domain of a cellulose-sensing trans-membrane anti-sigma factor fromClostridium thermocellum.Biomol. NMR Assign.In press. [PMID: 25682099] [Full text (Publisher website)]

2. Xingzhe Yao, Yan Xiao, Qiu Cui* and Yingang Feng* (2015) 1H, 15N and 13C resonance assignments of translationally-controlled tumor protein from photosynthetic microalgaNannochloropsis oceanica.Biomol. NMR Assign.In press. [PMID: 25680850] [Full text (Publisher website)]