科研团队 科研团队

最小化 最大化

        代谢物组学团队目前有工作人员14名,在读研究生9名,其中博士研究生5名,硕士研究生4名。已毕业博士研究生6名,硕士研究生12名(6名本所和6名联合培养)。

工作人员

   崔球 博士,研究员,团队负责人

   丁筱珂 硕士,团队秘书

   冯银刚 博士,研究员

   肖艳 博士,副研究员

   宋晓金 博士,副研究员

   刘亚君 博士,副研究员

   东升 博士,副助理研究员

   陈超 博士,博士后

   王彦超 博士,博士后

   王森 博士,博士后

   刘纪阳 学士,技术员

   封雪梅 学士,技术员

   赵长宇 学士,技术员

   周晓峰 硕士,技术员

硕博在读

桃李天下

崔球 崔球

最小化 最大化

崔球,博士,研究员,博士生导师,代谢物组学团队负责人

        崔球,中国科学院青岛生物能源与过程研究所研究员,博士生导师,代谢物组学团队负责人,中科院"百人计划"。1997年四川大学生物工程学士,2004年中科院生物物理所生物化学与分子生物学专业博士。2004至2008年于美国威斯康星大学麦迪逊分校生物化学系从事代谢物组学研究,2009年全职回国工作并获中科院"百人计划"资助。近年来在Nature Biotechnology、Biotechnology for Biofuels、J. Biol. Chem、Proteins、Biochemistry等著名学术期刊发表论文数十篇。主要研究领域是基于代谢物组/代谢流组学的计算分析辅助能源微生物代谢工程设计、蛋白质结构功能研究。

发表论文:

1.        Ya‑Jun Liu†*, Shiyue Liu†, Sheng Dong, Renmin Li, Yingang Feng*, and Qiu Cui* (2018) Determination of the native features of the exoglucanase Cel48S from Clostridium thermocellum. Biotechnol. Biofuels 11, 6. (*通讯作者)

2.        Huidan Zhang, Yingang Feng, Qiu Cui*, and Xiaojin Song* (2017) Expression of Vitreoscilla hemoglobin enhances production of arachidonic acid and lipids in Mortierella alpine. BMC Biotechnol. 17, 68. (*通讯作者)

3.        Lei Du†, Sheng Dong†, Xingwang Zhang, Chengying Jiang, Jingfei Chen, Lishan Yao, Xiao Wang, Xiaobo Wan, Xi Liu, Xinquan Wang, Shaohua Huang, Qiu Cui, Yingang Feng*, Shuang-Jiang Liu*, and Shengying Li* (2017) Selective oxidation of aliphatic C-H bonds in alkylphenols by a chemomimetic biocatalytic system. Proc. Natl. Acad. Sci. USA114(26):E5129-E5137.

4.        Zengxin Ma, Miaomiao Tian, Yanzhen Tan, Guzhen Cui, Yingang Feng, Qiu Cui, and Xiaojin Song* (2017) Response mechanism of the docosahexaenoic acid producer Aurantiochytrium under cold stress. Algal Res. 25, 191-199.

5.        Jie Zhang, Shiyue Liu, Renmin Li, Wei Hong, Yan Xiao, Yingang Feng, Qiu Cui* and Ya‑Jun Liu* (2017) Efficient whole-cell-catalyzing cellulose saccharification using engineered Clostridium thermocellum. Biotechnol. Biofuels 10, 124. (*通讯作者)

6.        Patrick K. Chaffey†, Xiaoyang Guan†, Chao Chen, Yuan Ruan, Xingfeng Wang, Amy H. Tran, Theo N. Koelsch, Qiu Cui, Yingang Feng*, and Zhongping Tan* (2017) Structural insight into the stabilizing effect of O-glycosylation. Biochemistry 56(23), 2897-2906.

7.        Xiaojin Song, Zengxin Ma, Yanzhen Tan, Huidan Zhang, Qiu Cui* (2017) Wastewater recycling technology for fermentation in polyunsaturated fatty acid production.Bioresour. Technol. 235,79-86. (*通讯作者)

8.        Yu-rong Cheng, Zhi-jie Sun*, Gu-zhen Cui, Xiaojin Song, Qiu Cui* (2016) A new strategy for strain improvement of Aurantiochytrium sp. based on heavy-ions mutagenesis and synergistic effects of cold stress and inhibitors of enoyl-ACP reductase. Enzyme Microbiol. Technol. 93-94,182-190. (*通讯作者)

9.        Gu-Zhen Cui, Zengxin Ma, Ya-Jun Liu, Yingang Feng, Zhijie Sun, Yurong Cheng, Xiaojin Song*, Qiu Cui (2016) Overexpression of glucose-6-phosphate dehydrogenase enhanced the polyunsaturated fatty acid composition of Aurantiochytrium sp. SD116. Algal Res. 19, 138-145.

10.    Chen Wang, Jinsong Xuan*, Qiu Cui, Yingang Feng* (2016) Resonance assignments of a VapC family toxin from Clostridium thermocellum. Biomol. NMR Assign.10(2), 367-371.

11.    Ya-Jun Liu, Xiaxia Song, Yifei Li, Jinsong Xuan, Qiu Cui, Jinfeng Wang, Yingang Feng* (2016) Low stability of the reduced state of Mycobacterium tuberculosis NrdH redoxin. FEBS Lett. 590(3), 387-395.

12.    Chao Chen, Zhenling Cui, Xiangfei Song, Ya-Jun Liu, Qiu Cui*, and Yingang Feng* (2016) Integration of bacterial expansin-like proteins into cellulosome promotes the cellulose degradation. Appl. Microbiol. Biotechnol. 100(5), 2203-2212.(*通讯作者)

13.    Zengxin Ma, Yanzhen Tan, Guzhen Cui, Yingang Feng, Qiu Cui* and Xiaojin Song* (2015) Transcriptome and gene expression analysis of DHA producer Aurantiochytrium under low temperature conditions. Sci. Rep. 5, 14446. (*通讯作者)

14.    Xiaojin Song*, Xiaolong Wang, Yanzhen Tan, Yingang Feng, Wenli Li and Qiu Cui* (2015) High production of squalene using a newly isolated yeast-like strain Pseudozyma sp. SD301. J. Agric. Food Chem. 63(38), 8445-4851.(*通讯作者)

15.    Yan Xiao, Jingtao Zhang, Jiatao Cui, Xingzhe Yao, Zhijie Sun, Yingang Feng* and Qiu Cui* (2015) Simultaneous accumulation of neutral lipids and biomass in Nannochloropsis oceanica IMET1 under high light intensity and nitrogen replete conditions. Algal Res. 11, 55-62. (*通讯作者)

16.    Jie Zhang, Ya-Jun Liu*, Gu-Zhen Cui and Qiu Cui* (2015) A novel arabinose-inducible genetic operation system developed for Clostridium cellulolyticum. Biotechnol. Biofuels 8, 36. (*通讯作者)

17.    Ya-Jun Liu, Jie Zhang, Gu-Zhen Cui and Qiu Cui* (2015) Current progress of targetron technology: Development, improvement and application in metabolic engineering.Biotechnol. J. 10(6), 855-865. (*通讯作者)

18.    Xiaoke Ding, Chao Chen, Qiu Cui, Wenli Li* and Yingang Feng* (2015) Resonance assignments of the periplasmic domain of a cellulose-sensing trans-membrane anti-sigma factor from Clostridium thermocellum. Biomol. NMR Assign. In press.

19.    Xingzhe Yao, Yan Xiao,Qiu Cui* and Yingang Feng* (2015) 1H, 15N and 13C resonance assignments of translationally-controlled tumor protein from photosynthetic microalga Nannochloropsis oceanica. Biomol. NMR Assign. 9(2), 325-328. (*通讯作者)

20.    兰君,宋晓金,谭延振,徐建春,,冯银刚* (2015) 利用伯克霍尔德氏菌脂肪酶富集裂壶藻油脂中的DHA。食品科学36(1), 128-134.

21.    Chao Chen, Zhenling Cui, Yan Xiao, Qiu Cui, Steven P. Smith, Raphael Lamed, Edward A. Bayer and Yingang Feng* (2014) Revisiting the NMR solution structure of the Cel48S type-I dockerin module from Clostridium thermocellum reveals a cohesin-primed conformation. J. Struct. Biol. 188(2), 188-193.

22.    兰君,谭延振,宋晓金,崔球,冯银刚* (2014) 裂殖壶菌SD116油脂的胞内核磁共振研究。生物物理学报30(3), 193-206.

23.    朱新术,崔家涛,冯银刚,张景涛*,崔球* (2014) 热纤梭菌中心代谢产物的归属及初步代谢组分析。波谱学杂志31(2), 243-252. (*通讯作者)

24.    Wei Hong, Jie Zhang, Yingang Feng, Georg Mohr, Alan M. Lambowitz, Gu-Zhen Cui, Ya-Jun Liu*, Qiu Cui* (2014) The contribution of cellulosomal scaffoldins to cellulose hydrolysis by Clostridium thermocellum analyzed by using thermotargetrons. Biotechnol. Biofuels 7, 80. (*通讯作者)

25.    Yingang Feng*, Xiaxia Song, Jinzhong Lin, Jinsong Xuan, Qiu Cui, Jinfeng Wang (2014) Structure determination of archaea-specific ribosomal protein L46a reveals a novel protein fold. Biochem. Biophys. Res. Commun. 450(1), 67-72.

26.    刘亚君,崔古贞,洪伟,张杰,崔球*(2014) 典型产纤维小体梭菌的遗传改造及其在纤维素乙醇中的应用研究进展。生物加工过程,第12卷第1期,55-62. (*通讯作者)

27.    Gu-Zhen Cui, Jie Zhang, Wei Hong, Chenggang Xu, Yingang Feng, Qiu Cui*, Ya-Jun Liu* (2014) Improvement of ClosTron for successive gene disruption in Clostridium cellulolyticum using a pyrF-based screening system. App. Microbiol. Biotechnol.98(1), 313-323. (*通讯作者)

28.    崔家涛,谭延振,崔球,宋晓金* (2013) 海洋真菌SD116在产物合成中的辅因子谱分析。中国海洋药物,第32卷第6期,27-32.

29.    Xiaojin Song*†, Yanzhen Tan†, Yajun Liu, Jingtao Zhang, Guanglei Liu, Yingang Feng, and Qiu Cui* (2013) Different impact of short-chain fatty acids on saturated and polyunsaturated fatty acid biosynthesis in Aurantiochytrium sp. SD116. J. Agric. Food Chem. 61(41), 9876-9881. (*通讯作者)

30.    Jiatao Cui, Jingtao Zhang, Xinshu Zhu, Fali Bai, Yingang Feng, Wenna Guan*, and Qiu Cui* (2013) Separation and quantification of water-soluble cellular metabolites in Clostridium thermocellum using liquid chromatography-isotope dilution tandem mass spectrometry. Anal. Lett. 46(17), 2767-2786. (*通讯作者)

31.    Xinshu Zhu, Jiatao Cui, Yingang Feng, Yun Fa, Jingtao Zhang*,Qiu Cui* (2013) Metabolic Adaption of Ethanol-Tolerant Clostridium thermocellum. PLoS ONE 8(7), e70631. (*通讯作者)

32.    Georg Mohr, Wei Hong, Jie Zhang, Gu-zhen Cui, Yunfeng Yang, Qiu Cui, Ya-jun Liu*, Alan M. Lambowitz* (2013) A targetron system for gene targeting in thermophiles and its Application in Clostridium thermocellum. PLoS ONE 8(7), e69032.

33.    Tong Li, Yuanyuan Du, Qiu Cui, Jingtao Zhang, Weiming Zhu, Kui Hong and Wenli Li* (2013) Cloning, characterization and heterologous expression of the indolocarbazole biosynthetic gene cluster from marine-derived Streptomyces sanyensis FMA. Mar. Drugs 11(2), 466-488.

34.    Lu Lin, Yuetong Ji, Qichao Tu, Ranran Huang, Lin Teng, Xiaowei Zeng, Houhui Song, Kun Wang, Qian Zhou, Yifei Li, Qiu Cui, Zhili He, Jizhong Zhou and Jian Xu* (2103) Microevolution from shock to adaptation revealed strategies improving ethanol tolerance and production in Thermoanaerobacter. Biotechnol. Biofuels 6,103.

35.    Mang Gao, Xiaojin Song*, Yingang Feng, Wenli Li, and Qiu Cui (2013) Isolation and characterization of Aurantiochytrium species: high docosahexaenoic acid (DHA) production by the newly isolated microalga, Aurantiochytrium sp. SD116. J. Oleo Sci. 62(3), 143-151.

36.    Yan Xiao, Jingtao Zhang, Jiatao Cui, Yingang Feng,Qiu Cui* (2013) Metabolic profiles of Nannochloropsis oceanica IMET1 under nitrogen-deficiency stress. Bioresour. Technol. 130, 731-738. (*通讯作者)

37.    Zhenling Cui, Yifei Li*, Yan Xiao, Yingang Feng, and Qiu Cui* (2013) Resonance assignments of cohesin and dockerin domains from Clostridium acetobutylicum ATCC824. Biomol. NMR Assign. 7(1), 73-76.(*通讯作者)

38.    Shan Wang, Gu-Zhen Cui, Xiang-Fei Song, Yingang Feng* and Qiu Cui* (2012) Efficiency and stability enhancement of cis-epoxysuccinic acid hydrolase by fusion with a carbohydrate binding module and immobilization onto cellulose. Appl. Biochem. Biotechnol. 168, 708-717. (*通讯作者)

39.    Gu-Zhen Cui, Shan Wang, Yifei Li, Yi-Jun Tian, Yingang Feng, and Qiu Cui* (2012) High yield recombinant expression, characterization and homology modeling of two types of cis-epoxysuccinic acid hydrolases. Protein J. 31(5), 432-438. (*通讯作者)

40.    Gu-zhen Cui, Wei Hong, Jie Zhang, Wen-li Li, Yingang Feng, Ya-jun Liu*, and Qiu Cui* (2012) Targeted gene engineering in Clostridium cellulolyticum H10 without methylation. J. Microbiol. Methods 89(3), 201-208. (*通讯作者)

41.    Jitian Liu, Yu Tang*, Kaigui Wu, Caifeng Bi*, and Qiu Cui* (2012) Conversion of fructose into 5-hydroxymethylfurfural (HMF) and its derivatives promoted by inorganic salt in alcohol.Carbohydr. Res. 350, 20-24. (*通讯作者)

42.    Xianyang Fang†,Qiu Cui†, Yufeng Tong, Yingang Feng, Lu Shan, Li Huang, Jinfeng Wang* (2008) A stabilizing alpha/beta-hydrophobic core greatly contributes to hyperthermostability of archaeal [P62A]Ssh10b. Biochemistry 47(43), 11212-11221.(†共同第一作者)

43.    Qiu Cui, Ian A. Lewis, Adrian D. Hegeman, Mark E. Anderson, Jing Li, Christopher F. Schulte, William M. Westler, Hamid R. Eghbalnia, Michael R. Sussman & John L. Markley (2008) Metabolite identification via the Madison Metabolomics Consortium Database. Nature Biotechnol. 2008, 26(2), 162-164.

44.    John L. Markley*, Mark E. Anderson, Qiu Cui, Hamid R. Eghbalnia, Ian A. Lewis, Adrian D. Hegeman, Jing Li, Christopher F. Schulte, Michael R. Sussman, William M. Westler, Eldon L. Ulrich, Zsolt Zolnai (2007): New bioinformatics resources for metabolomics.Pac. Symp. Biocomput. 12, 157-168.

45.    Adrian D. Hegeman, Christopher F. Schulte, Qiu Cui, Ian A. Lewis, Edward L. Huttlin, Hamid Eghbalnia, Amy C. Harms, Eldon L. Ulrich, John L. Markley, and Michael R. Sussman (2007) Stable Isotope Assisted Assignment of Elemental Compositions for Metabolomics. Anal. Chem. 79(18), 6912-6921.

46.    Qiu Cui†, Michael P. Thorgersen†, William M. Westler, John L. Markley, Diana M. Downs* (2006) Solution structure of YggX: a prokaryotic protein involved in Fe(II) trafficking. Proteins 62(3), 578-586. (†共同第一作者)

47.    Qiu Cui, Yufeng Tong, Hong Xue, Li Huang, Yingang Feng and Jinfeng Wang* (2003) Two conformations of archaeal Ssh10b: the origin of its temperature dependent interaction with DNA.  J. Biol. Chem. 278(51), 51015-51022.

48.    Yufeng Tong, Qiu Cui, Yingang Feng, Li Huang and Jinfeng Wang* (2002) Letters to the Editor: 1H, 15N, and 13C resonance assignments and secondary structure of the Ssh10b from hyperthermophilic archaeon Sulfolobus shibatae. J. Biomol. NMR, 22(4), 385-386.

主持基金项目(纵向)

1.      国家重点研发计划:近海生态系统碳汇形成过程与机理(2016.7-2021.6)

2.      中科院项目:木质纤维素动物营养素(2016.1-2020.12)

3.      国家自然科学基金面上项目:热纤梭菌纤维小体的产物抑制机理及工程改造(2015-2018)

4.      中科院科技服务网络计划:多不饱和脂肪酸DHA和衣康酸工业生产菌株改造及产业应用(2015-2016)

5.      中科院科研研制装备项目:基于超声空化及电穿孔联合作用的通用细胞基因转移系统(2012-2013)

6.      973课题:细菌酶复合体纤维素降解转化机理和纤维小体的解析、重构(2011-2015)

7.      国家自然科学基金面上项目:超快速动态代谢物组学分析在热纤梭菌纤维素降解中的应用(2010-2012)

8.      中国科学院百人项目:高温乙醇菌系统生物学改造的关键代谢物学技术(2009-2011)

9.      中国科学院信息化专项:能源微生物功能基因组的整合分析数据库(2009-2010)

10.  中国科学院知识创新项目:基于全基因组的高产油微藻代谢功能比较及遗传体系构建研究  (2009-2010)

11.  中科院重要方向项目:能源微生物网络实验室(2009-2010)